Boster Immunoleader

antibody

Anti-TREM1 Antibody

PA1586

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-TREM1 Antibody

PA1586

Polyclonal

Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1(TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle of mouse TREM1(74-90aa FTRPSEVHMGKFTLKHD).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

1. Allcock, R. J. N., Barrow, A. D., Forbes, S., Beck, S., Trowsdale, J.The human TREM gene cluster at 6p21.1 encodes both activating and inhibitory single IgV domain receptors and includes NKp44.Europ. J. Immun. 33: 567-577, 2003. 2. Bouchon, A., Dietrich, J., Colonna, M.Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes.J. Immun. 164: 4991-4995, 2000.

TREM1

Triggering receptor expressed on myeloid cells 1(TREM-1)

triggering receptor expressed on myeloid cells 1

CD354 antibody OTTMUSP00000018206 antibody TREM 1 antibody TREM-1 antibody TREM1 antibody TREM1_HUMAN antibody Triggering receptor expressed on monocytes 1 antibody Triggering receptor expressed on myeloid cells 1 antibody Triggering receptor TREM 1 antibody Triggering receptor TREM1 antibody

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