Boster Immunoleader

antibody

Anti-GRB2 Antibody

PA1579

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-GRB2 Antibody

PA1579

Polyclonal

Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 2(GRB2) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human GRB2(184-201aa HVMDNSDPNWWKGACHGQ), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

1. Cheng, A. M., Saxton, T. M., Sakai, R., Kulkarni, S., Mbamalu, G., Vogel, W., Tortorice, C. G., Cardiff, R. D., Cross, J. C., Muller, W. J., Pawson, T.Mammalian Grb2 regulates multiple steps in embryonic development and malignant transformation.Cell 95: 793-803, 1998. 2. Clark, S. G., Stern, M. J., Horvitz, H. R.C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains.Nature 356: 340-344, 1992. 3. Hill, R. J., Zozulya, S., Lu, Y.-L., Ward, K., Gishizky, M., Jallal, B.The lymphoid protein tyrosine phosphatase Lyp interacts with the adaptor molecule Grb2 and functions as a negative regulator of T-cell activation.Exp. Hemat. 30: 237-244, 2002.

GRB2

Growth factor receptor-bound protein 2

growth factor receptor-bound protein 2

Abundant SRC homology antibody Adapter protein GRB2 antibody ASH antibody Ash protein antibody EGFRBP GRB2 antibody Epidermal growth factor receptor binding protein antibody Epidermal growth factor receptor binding protein GRB2 antibody GRB 2 antibody GRB2 adapter protein antibody Grb2 antibody GRB2_HUMAN antibody Grb3 3 antibody Growth Factor Receptor Bound Protein 2 antibody Growth factor receptor bound protein 3 antibody Growth factor receptor-bound protein 2 antibody HT027 antibody MST084 antibody MSTP084 antibody NCKAP2 antibody OTTHUMP00000166096 antibody OTTHUMP00000166097 antibody OTTHUMP00000166098 antibody Protein ASH antibody SEM5 antibody SH2/SH3 adapter GRB2 antibody

P62993