Boster Immunoleader

antibody

Anti-Fas Ligand Antibody

PA1576

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Fas Ligand Antibody

PA1576

Polyclonal

Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(263-281aa ELSLVNFEESQTFFGLYKL).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

1. Asanuma, K., Tsuji, N., Endoh, T., Yagihashi, A., Watanabe, N.Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells.J. Immun. 172: 3922-3929, 2004. 2. Hahne, M., Rimoldi, D., Schroter, M., Romero, P., Schreier, M., French, L. E., Schneider, P., Bornand, T., Fontana, A., Lienard, D., Cerottini, J. C., Tschopp, J.Melanoma cell expression of Fas (Apo-1/CD95) ligand: implications for tumor immune escape.Science 274: 1363-1366, 1996. 3. Nakamura, T., Imai, Y., Matsumoto, T., Sato, S., Takeuchi, K., Igarashi, K., Harada, Y., Azuma, Y., Krust, A., Yamamoto, Y., Nishina, H., Takeda, S., Takayanagi, H., Metzger, D., Kanno, J., Takaoka, K., Martin, T. J., Chambon, P., Kato, S.Estrogen prevents bone loss via estrogen receptor alpha and induction of Fas ligand in osteoclasts.Cell 130: 811-823, 2007.

FASLG

Tumor necrosis factor ligand superfamily member 6

Fas ligand(TNF superfamily, member 6)

ALPS1B antibody APO1L antibody Apoptosis(APO 1) antigen ligand 1 antibody Apoptosis antigen ligand 1 antibody Apoptosis antigen ligand antibody APT1LG1 antibody APTL antibody CD178 antibody CD178 antigen antibody CD95 ligand antibody CD95-L antibody CD95L antibody CD95L protein antibody FAS antigen ligand antibody Fas L antibody Fas ligand(TNF superfamily member 6) antibody Fas ligand antibody FASL antibody Fasl Fas ligand(TNF superfamily member 6) antibody FASLG antibody Generalized lymphoproliferative disease antibody Gld antibody soluble form antibody TNF superfamily member 6 antibody TNFL6_HUMAN antibody TNFSF6 antibody Tumor necrosis factor(ligand) superfamily member 6 antibody Tumor necrosis factor ligand superfamily member 6 antibody

P48023