Anti-Cdc25A Antibody | Boster Immunoleader PA1545 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cdc25A Antibody

catalog :

PA1545

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cdc25A Antibody

Catalog Number :

PA1545

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 1(CDC25A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Cdc25A(1-17aa MELGPEPPHRRRLLFAC), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

The human CDC25 tyrosine phosphatases trigger activation of CDC2 by removing inhibitory phosphate from tyrosine and threonine residues of the cyclin-dependent kinases. The genes encoding these phosphatases are suspected of being potential oncogenes because of their role in promoting cell division. Three human CDC25 genes have been identified: CDC25A, CDC25B, and CDC25C. The CDC25A gene is mapped to 3p21. In rodent cells, human CDC25A or CDC25B but not CDC25C phosphatases cooperate with either the gly12-to-val mutation of the HRAS gene or loss of RB1 in oncogenic focus formation.

Reference :

1. Demetrick, D. J., Beach, D. H.Chromosome mapping of human CDC25A and CDC25B phosphatases.Genomics 18: 144-147, 1993. 2. Galaktionov, K., Lee, A. K., Eckstein, J., Draetta, G., Meckler, J., Loda, M., Beach, D.CDC25 phosphatases as potential human oncogenes.Science 269: 1575-1577, 1995.

Gene Name :

CDC25A

Protein Name :

M-phase inducer phosphatase 1

Gene Full Name :

cell division cycle 25 homolog A(S. pombe)

Synonyms :

Cdc 25a antibody CDC25A antibody CDC25A1 antibody CDC25A2 antibody CDC25A2 CAG isoform antibody Cell division cycle 25 homolog A(S. pombe) antibody Cell division cycle 25A antibody Cell division cycle 25A isoform a antibody Cell division cycle 25A isoform b antibody D9Ertd393e antibody Dual specificity phosphatase Cdc25A antibody M phase inducer phosphatase 1 antibody M-phase inducer phosphatase 1 antibody MGC115549 antibody MPIP1_HUMAN antibody

Uniprot ID :

P30304

more info or order :

Boster Immunoleader product webpage