Boster Immunoleader

antibody

Anti-BCAT1 Antibody

PA1538

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-BCAT1 Antibody

PA1538

Polyclonal

Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1(360-375aa KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

1. Naylor, S. L., Shows, T. B.Branched-chain aminotransferase genes (BCT-1 and BCT-2) assigned to human chromosomes 12 and 19 using alpha-keto acid selection media. (Abstract)Cytogenet. Cell Genet. 25: 191-192, 1979. 2. Jones, C., Moore, E. E.Isolation of mutants lacking branched-chain amino acid transaminase.Somat. Cell Genet. 2: 235-243, 1976.

BCAT1

Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c))

branched chain amino-acid transaminase 1, cytosolic

BCAT antibody BCAT(c) antibody Bcat1 antibody BCAT1_HUMAN antibody BCT1 antibody Branched chain amino acid aminotransferase, cytosolic antibody branched chain amino-acid transaminase 1, cytosolic antibody branched chain aminotransferase 1 antibody branched chain aminotransferase 1, cytosolic antibody Branched-chain-amino-acid aminotransferase antibody cytosolic antibody DKFZp686E12175 antibody EC 2.6.1.42 antibody ECA39 antibody MECA39 antibody placental protein 18 antibody PNAS 121 antibody PP18 antibody Protein ECA39 antibody

P54687