Boster Immunoleader

antibody

Anti-ATF4 Antibody

PA1537

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-ATF4 Antibody

PA1537

Polyclonal

Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-4(ATF4) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4(332-351aa QYLKDLIEEVRKARGKKRVP), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

ATF4, Activating Transcription Factor 4, is also know as CREB2. ATF4 belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. It is identified that members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. The ATF4 gene is mapped to chromosome 22. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the CREB2 protein.

1. De Angelis, R., Iezzi, S., Bruno, T., Corbi, N., Di Padova, M., Floridi, A., Fanciulli, M., Passananti, C.Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4).FEBS Lett. 547: 15-19, 2003. 2. Hai, T., Liu, F., Coukos, W. J., Green, M. R.Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.Genes Dev. 3: 2083-2090, 1989. 3. Karpinski, B. A., Morle, G. D., Huggenvik, J., Uhler, M. D., Leiden, J. M.Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element.Proc. Nat. Acad. Sci. 89: 4820-4824, 1992.

ATF4

Cyclic AMP-dependent transcription factor ATF-4(cAMP-dependent transcription factor ATF-4)

activating transcription factor 4(tax-responsive enhancer element B67)

Activating Transcription Factor 4 antibody ATF 4 antibody ATF4 antibody ATF4 protein antibody ATF4_HUMAN antibody cAMP-dependent transcription factor ATF-4 antibody cAMP-responsive element-binding protein 2 antibody CREB 2 antibody CREB-2 antibody CREB2 antibody Cyclic AMP dependent transcription factor ATF 4 antibody Cyclic AMP response element binding protein 2 antibody Cyclic AMP-dependent transcription factor ATF-4 antibody Cyclic AMP-responsive element-binding protein 2 antibody DNA binding protein TAXREB67 antibody DNA-binding protein TAXREB67 antibody Tax Responsive Enhancer Element B67 antibody Tax-responsive enhancer element-binding protein 67 antibody TAXREB67 antibody TXREB antibody

P18848