Anti-Collagen IV Antibody | Boster Immunoleader PA1536-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Collagen IV Antibody

catalog :

PA1536-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Collagen IV Antibody

Catalog Number :

PA1536-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Collagen IV(245-263aa QAQVQEKGDFATKGEKGQK), different from the related mouse and rat sequences by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Collagen alpha-1(IV) chain is a protein that in humans is encoded by the COL4A1 gene. Type IV collagen is the major structural component of glomerular basement membranes(GBM), forming a'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in COL4A1 exons 24 and 25 are associated with HANAC(autosomal dominant hereditary angiopathy with nephropathy,aneurysms, and muscle cramps).

Reference :

1. Mayne, R., Wiedemann, H., Irwin, M. H., Sanderson, R. D., Fitch, J. M., Linsenmayer, T. F., Kuhn, K.Monoclonal antibodies against chicken type IV and V collagens: electron microscopic mapping of the epitopes after rotary shadowing.J. Cell Biol. 98: 1637-1644, 1984. 2. Solomon, E., Hiorns, L. R., Spurr, N., Kurkinen, M., Barlow, D., Hogan, B. L. M., Dalgleish, R.Chromosomal assignments of the genes coding for human types II, III and IV collagen: a dispersed gene family.Proc. Nat. Acad. Sci. 82: 3330-3334, 1985. 3. Sudhakar, A., Nyberg, P., Keshamouni, V. G., Mannam, A. P., Li, J., Sugimoto, H., Cosgrove, D., Kalluri, R.Human alpha-1 type IV collagen NC1 domain exhibits distinct antiangiogenic activity mediated by alpha-1-beta-1 integrin.J. Clin. Invest. 115: 2801-2810, 2005.

Gene Name :

COL4A1

Protein Name :

Collagen alpha-1(IV) chain

Gene Full Name :

collagen, type IV, alpha 1

Synonyms :

Arresten antibody Canstatin antibody CO4A1_HUMAN antibody COL4A1 antibody COL4A1 NC1 domain antibody Collagen Alpha 1(IV) Chain antibody collagen alpha-1(IV) chain antibody Collagen IV Alpha 1 Polypeptide antibody Collagen Of Basement Membrane Alpha 1 Chain antibody Collagen Type IV Alpha 1 antibody DKFZp686I14213 antibody FLJ22259 antibody

Uniprot ID :

P02462

more info or order :

Boster Immunoleader product webpage