Boster Immunoleader

antibody

Anti-TATA binding protein TBP Antibody

PA1534

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

info changed 20150320

Anti-TATA binding protein TBP Antibody

PA1534

Polyclonal

Rabbit IgG polyclonal antibody for TATA-box-binding protein(TBP) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human TATA binding protein TBP(227-246aa EEQSRLAARKYARVVQKLGF), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.

1. Bauer, P., Laccone, F., Rolfs, A., Wullner, U., Bosch, S., Peters, H., Liebscher, S., Scheible, M., Epplen, J. T., Weber, B. H. F., Holinski-Feder, E., Weirich-Schwaiger, H., Morris-Rosendahl, D. J., Andrich, J., Riess, O.Trinucleotide repeat expansion in SCA17/TBP in white patients with Huntington's disease-like phenotype.J. Med. Genet. 41: 230-232, 2004. 2. Stevanin, G., Fujigasaki, H., Lebre, A.-S., Camuzat, A., Jeannequin, C., Dode, C., Takahashi, J., San, C., Bellance, R., Brice, A., Durr, A.Huntington's disease-like phenotype due to trinucleotide repeat expansions in the TBP and JPH3 genes.Brain 126: 1599-1603, 2003. 3. Veenstra, G. J. C., Weeks, D. L., Wolffe, A. P.Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus.Science 290: 2312-2314, 2000.

TBP

TATA-box-binding protein

TATA box binding protein

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P20226