Anti-Caspase-1(P10) Antibody | Boster Immunoleader PA1522 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Caspase-1(P10) Antibody

catalog :

PA1522

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, et al. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. Int J Mol Med. 2015;35:1179-88 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Caspase-1(P10) Antibody

Catalog Number :

PA1522

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Caspase-1(CASP1) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-1(P10)(249-262aa VFIGRLIEHMQEYA).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Caspase 1, apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. Caspase 1 was purified ICE from the cytosol of the THP. human monocytic cell line and found that the active protease was made up of 2 peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Reference :

1. Cerretti, D. P., Hollingsworth, L. T., Kozlosky, C. J., Valentine, M. B., Shapiro, D. N., Morris, S. W., Nelson, N.Molecular characterization of the gene for human interleukin-1-beta converting enzyme (IL1BC).Genomics 20: 468-473, 1994. 2. Thornberry, N. A., Bull, H. G., Calaycay, J. R., Chapman, K. T., Howard, A. D., Kostura, M. J., Miller, D. K., Molineaux, S. M., Weidner, J. R., Aunins, J., Elliston, K. O., Ayala, J. M., Casano, F. J., Chin, J., Ding, G. J.-F., Egger, L. A., Gaffney, E. P., Limjuco, G., Palyha, O. C., Raju, S. M., Rolando, A. M., Salley, J. P., Yamin, T.-T., Lee, T. D., Shively, J. E., MacCross, M., Mumford, R. A., Schmidt, J. A., Tocci, M. J.A novel heterodimeric cysteine protease is required for interleukin-1-beta processing in monocytes.Nature 356: 768-774, 1992.

Gene Name :

CASP1

Protein Name :

Caspase-1(CASP-1)

Gene Full Name :

caspase 1, apoptosis-related cysteine peptidase

Synonyms :

CASP 1 antibody CASP-1 antibody CASP1 antibody CASP1_HUMAN antibody Caspase 1 apoptosis related cysteine peptidase antibody Caspase 1 apoptosis related cysteine protease antibody Caspase-1 subunit p10 antibody Caspase1 antibody ICE antibody IL 1 beta converting enzyme antibody IL 1BC antibody IL-1 beta-converting enzyme antibody IL-1BC antibody IL1 beta converting enzyme antibody IL1B convertase antibody IL1BC antibody IL1BCE antibody Interleukin 1 beta convertase antibody Interleukin 1 beta convertase precursor antibody Interleukin 1 beta converting enzyme antibody Interleukin 1B converting enzyme antibody Interleukin-1 beta convertase antibody Interleukin-1 beta-converting enzyme antibody P45 antibody

Uniprot ID :

P29466

more info or order :

Boster Immunoleader product webpage