Boster Immunoleader

antibody

Anti-CD68 Antibody

PA1518

100 ug/vial

200 USD

polyclonal

rabbit

mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

total: 13

1. Wang H, Zhang Q, Chai Y, Liu Y, Li F, Wang B, et al. 1,25(OH)2D 3 downregulates the Toll-like receptor 4-mediated inflammatory pathway and ameliorates liver injury in diabetic rats. J Endocrinol Invest. 2015;: pubmed
2. Gu X, Wu G, Yao Y, Zeng J, Shi D, Lv T, et al. Intratracheal administration of mitochondrial DNA directly provokes lung inflammation through the TLR9-p38 MAPK pathway. Free Radic Biol Med. 2015;83:149-58 pubmed publisher
3. Li N, Qin J, Lan L, Zhang H, Liu F, Wu Z, et al. PTEN inhibits macrophage polarization from M1 to M2 through CCL2 and VEGF-A reduction and NHERF-1 synergism. Cancer Biol Ther. 2015;16:297-306 pubmed publisher
4. Zheng T, Yang X, Wu D, Xing S, Bian F, Li W, et al. Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3β pathway. Br J Pharmacol. 2015;: pubmed publisher
5. He W, Lv Y, Zhao Y, Xu C, Jin Z, Qin C, et al. Core-shell structured gel-nanocarriers for sustained drug release and enhanced antitumor effect. Int J Pharm. 2015;484:163-71 pubmed publisher
6. Liu Z, Xu S, Huang X, Wang J, Gao S, Li H, et al. Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1). Br J Pharmacol. 2015;: pubmed publisher
7. Wang W, Li X, Zheng D, Zhang D, Peng X, Zhang X, et al. Dynamic changes and functions of macrophages and M1/M2 subpopulations during ulcerative colitis-associated carcinogenesis in an AOM/DSS mouse model. Mol Med Rep. 2015;11:2397-406 pubmed publisher
8. Pan X, Wang P, Luo J, Wang Z, Song Y, Ye J, et al. Adipogenic changes of hepatocytes in a high-fat diet-induced fatty liver mice model and non-alcoholic fatty liver disease patients. Endocrine. 2015;48:834-47 pubmed publisher
9. Zhang K, Liu Z, Li G, Lai B, Qin L, Ding Y, et al. Electro-acupuncture promotes the survival and differentiation of transplanted bone marrow mesenchymal stem cells pre-induced with neurotrophin-3 and retinoic acid in gelatin sponge scaffold after rat spinal cord transection. Stem Cell Rev. 2014;10:612-25 pubmed publisher
10. Zhu F, Zhang L, Ding Y, Zhao J, Zheng Y. Neonatal intrahippocampal injection of lipopolysaccharide induces deficits in social behavior and prepulse inhibition and microglial activation in rats: Implication for a new schizophrenia animal model. Brain Behav Immun. 2014;38:166-74 pubmed publisher

Anti-CD68 Antibody

PA1518

Polyclonal

Rabbit IgG polyclonal antibody for Macrosialin(CD68) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of mouse CD68(312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

1. Holness, C. L., Simmons, D. L.Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.Blood 81: 1607-1613, 1993. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R.The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13.Genomics 53: 248-250, 1998.

CD68

Macrosialin

CD68 molecule

CD 68 antibody CD68 antibody CD68 antigen antibody CD68 molecule antibody CD68_HUMAN antibody DKFZp686M18236 antibody GP110 antibody LAMP4 antibody Macrophage antigen CD68(microsialin) antibody MACROPHAGE ANTIGEN CD68 antibody macrosialin antibody SCARD1 antibody Scavenger receptor class D member 1 antibody

P31996