Anti-Caspase-10 Antibody | Boster Immunoleader PA1513 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Caspase-10 Antibody

catalog :

PA1513

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Caspase-10 Antibody

Catalog Number :

PA1513

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Caspase-10(CASP10) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CASP10(220-236aa VKTFLEALPRAAVYRMN).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. The Caspase 10 gene contains 11 exons and spans about 48 kb. This gene is mapped to 2q33.1. It is transcribed in the centromere-to-telomere direction.This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Reference :

1. Lee, H.-J., Pyo, J.-O., Oh, Y., Kim, H.-J., Hong, S., Jeon, Y.-J., Kim, H., Cho, D.-H., Woo, H.-N., Song, S., Nam, J.-H., Kim, H. J., Kim, K.-S., Jung, Y.-K.AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10.Nature Cell Biol. 9: 1303-1310, 2007. 2. Park, W. S., Lee, J. H., Shin, M. S., Park, J. Y., Kim, H. S., Lee, J. H., Kim, Y. S., Lee, S. N., Xiao, W., Park, C. H., Lee, S. H., Yoo, N. J., Lee, J. Y.Inactivating mutations of the caspase-10 gene in gastric cancer.Oncogene 21: 2919-2925, 2002. 3. Wang, J., Zheng, L., Lobito, A., Chan, F. K., Dale, J., Sneller, M., Yao, X., Puck, J. M., Straus, S. E., Lenardo, M. J.Inherited human caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II.Cell 98: 47-58, 1999.

Gene Name :

CASP10

Protein Name :

Caspase-10(CASP-10)

Gene Full Name :

caspase 10, apoptosis-related cysteine peptidase

Synonyms :

ALPS 2 antibody ALPS2 antibody Apoptosis related cysteine peptidase antibody Apoptotic protease Mch 4 antibody Apoptotic protease Mch-4 antibody CASP 10 antibody CASP-10 antibody CASP10 antibody CASP10 protein antibody CASPA_HUMAN antibody Caspase 10 apoptosis related cysteine peptidase antibody Caspase 10 apoptosis related cysteine protease antibody Caspase-10 subunit p12 antibody Caspase10 antibody FADD Like Ice 2 antibody FADD like ICE2 antibody Fas Associated Death Domain Protein antibody FAS associated death domain protein interleukin 1B converting enzyme 2 antibody FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody FLICE 2 antibody FLICE2 antibody ICE Like Apoptotic Protease 4 antibody ICE-like apoptotic protease 4 antibody Interleukin 1B Converting Enzyme 2 antibody MCH 4 antibody MCH4 antibody

Uniprot ID :

Q92851

more info or order :

Boster Immunoleader product webpage