Boster Immunoleader

antibody

Anti-Bone Sialoprotein Antibody

PA1505

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Bone Sialoprotein Antibody

PA1505

Polyclonal

Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein(17-34aa FSMKNLHRRVKIEDSEEN), different from the related rat and mouse sequences by three amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

BSP is also known as IBSP(integrin-binding sialoprotein). The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

1. Duncan EL, et al. Genome-wide association study using extreme truncate selection identifies novel genes affecting bone mineral density and fracture risk. PLoS Genet, 2011 Apr. 2. Gordon JA, et al. Bone sialoprotein stimulates focal adhesion-related signaling pathways: role in migration and survival of breast and prostate cancer cells. J Cell Biochem, 2009 Aug 15.

IBSP

Bone sialoprotein 2

integrin-binding sialoprotein

BNSP antibody Bone sialoprotein 2 antibody Bone sialoprotein 2 precursor antibody Bone sialoprotein II antibody bone sialoprotein II; bone sialoprotein antibody BSP antibody BSP II antibody BSPII antibody Cell binding sialoprotein antibody Cell-binding sialoprotein antibody IBSP antibody Integrin binding sialoprotein antibody Integrin binding sialoprotein(bone sialoprotein II) antibody integrin binding sialoprotein(bone sialoprotein, bone sialoprotein II) antibody Integrin-binding sialoprotein antibody SIAL_HUMAN antibody SPII antibody

P21815