Anti-MCL1 Antibody | Boster Immunoleader PA1503 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MCL1 Antibody

catalog :

PA1503

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MCL1 Antibody

Catalog Number :

PA1503

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human MCL1(303-325aa RDWLVKQRGWDGFVEFFHVEDLE), different from the related mouse sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

BCL2L3, also known as MCL1(myeloid cell leukemia sequence 1) encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product(isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products(isoform 2 and isoform 3) promote apoptosis and are death-inducing. Using the methods of somatic cell hybrid analysis and fluorescence in situ hybridization), the MCL1 gene is mapped to human 1q21.MCL1is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex. The polyubiquitylation of MCL1 then targets it for proteasomal degradation. The degradation of MCL1 was blocked in patient-derived tumor cells that lacked FBW7 or had loss-of-function mutations in FBW7, conferring resistance to antitubulin agents and promoting chemotherapeutic-induced polyploidy.

Reference :

1. Inuzuka, H., Shaik, S., Onoyama, I., Gao, D., Tseng, A., Maser, R. S., Zhai, B., Wan, L., Gutierrez, A., Lau, A. W., Xiao, Y., Christie, A. L., Aster, J., Settleman, J., Gygi, S. P., Kung, A. L., Look, T., Nakayama, K. I., DePinho, R. A., Wei, W.SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction.Nature 471: 104-109, 2011. 2. Leu, J. I.-J., Dumont, P., Hafey, M., Murphy, M. E., George, D. L.Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nature Cell Biol. 6: 443-450, 2004. 3. Schwickart, M., Huang, X., Lill, J. R., Liu, J., Ferrando, R., French, D. M., Maecker, H., O'Rourke, K., Bazan, F., Eastham-Anderson, J., Yue, P., Dornan, D., Huang, D. C. S., Dixit, V. M.Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival.Nature 463: 103-107, 2010.

Gene Name :

MCL1

Protein Name :

Induced myeloid leukemia cell differentiation protein Mcl-1

Gene Full Name :

myeloid cell leukemia sequence 1(BCL2-related)

Synonyms :

Bcl 2 related protein EAT/mcl1 antibody Bcl-2-like protein 3 antibody Bcl-2-related protein EAT/mcl1 antibody BCL2 related antibody Bcl2-L-3 antibody BCL2L3 antibody EAT antibody Induced myeloid leukemia cell differentiation protein Mcl 1 antibody Induced myeloid leukemia cell differentiation protein Mcl-1 antibody MCL 1 antibody MCL1 antibody MCL1-ES antibody Mcl1/EAT antibody MCL1_HUMAN antibody MCL1L antibody MCL1S antibody MGC104264 antibody MGC1839 antibody Myeloid Cell Leukemia 1 antibody Myeloid cell leukemia ES antibody myeloid cell leukemia sequence 1 antibody Myeloid cell leukemia sequence 1 BCL2 related antibody Myeloid cell leukemia sequence 1 isoform 1 antibody OTTHUMP00000032794 antibody OTTHUMP00000032795 antibody TM antibody

Uniprot ID :

Q07820

more info or order :

Boster Immunoleader product webpage