Boster Immunoleader

antibody

Anti-CAMKK2 Antibody

PA1497

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-CAMKK2 Antibody

PA1497

Polyclonal

Rabbit IgG polyclonal antibody for Calcium/calmodulin-dependent protein kinase kinase 2(CAMKK2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse; Predicted Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human CAMKK2(180-194aa KLAYNENDNTYYAMK), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.

1. Cao W, Sohail M, Liu G, Koumbadinga GA, Lobo VG, Xie J.Differential effects of PKA-controlled CaMKK2 variants on neuronal differentiation. RNA Biol. 2011 Nov 1;8(6). 2. Teng EC, Racioppi L, Means AR. A cell-intrinsic role for CaMKK2 in granulocyte lineage commitment and differentiation. J Leukoc Biol. 2011 Aug 4. 3. Anderson KA, Ribar TJ, Lin F, Noeldner PK, Green MF, Muehlbauer MJ, Witters LA, Kemp BE, Means AR.Hypothalamic CaMKK2 contributes to the regulation of energy balance. Cell Metab. 2008 May;7(5):377-88.

CAMKK2

Calcium/calmodulin-dependent protein kinase kinase 2(CaM-KK 2/CaM-kinase kinase 2/CaMKK 2)

calcium/calmodulin-dependent protein kinase kinase 2, beta

Calcium/calmodulin dependent protein kinase beta antibody Calcium/calmodulin dependent protein kinase kinase 2 antibody Calcium/calmodulin dependent protein kinase kinase 2 beta antibody Calcium/calmodulin dependent protein kinase kinase beta antibody Calcium/calmodulin-dependent protein kinase kinase 2 antibody Calcium/calmodulin-dependent protein kinase kinase beta antibody CaM kinase kinase beta antibody CaM KK beta antibody CaM-kinase kinase 2 antibody CaM-kinase kinase beta antibody CaM-KK 2 antibody CaM-KK beta antibody CAMKK 2 antibody CAMKK antibody CaMKK beta antibody CAMKK beta protein antibody Camkk2 antibody CAMKKB antibody KKCC2_HUMAN antibody

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