Anti-APEX2 Antibody | Boster Immunoleader PA1495 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-APEX2 Antibody

catalog :

PA1495

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-APEX2 Antibody

Catalog Number :

PA1495

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase 2(APEX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human APEX2(504-518aa DPSSRCNFFLWSRPS), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

APEX2, also called apurinic/apyrimidinic endonuclease like-2, is a member of the apurinic/apyrimidinic(AP) family of endonucleases that initiate the repair of AP sites formed by spontaneous hydrolysis of the N-glycosylic bond, mutagen-induced base release, or damaged-base excision by a DNA repair glycosylase. RT-PCR detected APEX2 expression in HeLa cells, Jurkat cells, and human kidney, brain and fetal brain tissue. The APEX2 gene contains 6 exons. The APEX2 gene is mapped to chromosome Xp11.21. APEX2 participates in both nuclear and mitochondrial base excision repair(BER). APEX2 displayed weaker AP site-specific and 3-prime nuclease activities compared to APEX1. APEX2 plays a role in processing 3-prime-damaged termini or 3-prime-mismatched nucleotides.

Reference :

1. Burkovics, P., Szukacsov, V., Unk, I., Haracska, L.Human Ape2 protein has 3-5-prime exonuclease activity that acts preferentially on mismatched base pairs.Nucleic Acids Res. 34: 2508-2515, 2006. 2. Hadi, M. Z., Ginalski, K., Nguyen, L. H., Wilson, D. M., III.Determinants in nuclease specificity of Ape1 and Ape2, human homologues of Escherichia coli exonuclease III.J. Molec. Biol. 316: 853-866, 2002. 3. Tsuchimoto, D., Sakai, Y., Sakumi, K., Nishioka, K., Sasaki, M., Fujiwara, T., Nakabeppu, Y.Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen.Nucleic Acids Res. 29: 2349-2360, 2001

Gene Name :

APEX2

Protein Name :

DNA-(apurinic or apyrimidinic site) lyase 2

Gene Full Name :

APEX nuclease(apurinic/apyrimidinic endonuclease) 2

Synonyms :

AP endonuclease 2 antibody AP endonuclease XTH2 antibody APE 2 antibody APE2 antibody APEX 2 antibody APEX L2 antibody APEX nuclease(apurinic/apyrimidinic endonuclease) 2 antibody APEX nuclease 2 antibody APEX nuclease like 2 antibody APEX nuclease-like 2 antibody APEX2 antibody APEX2_HUMAN antibody APEXL 2 antibody APEXL2 antibody Apurinic apyrimidinic endonuclease 2 antibody Apurinic-apyrimidinic endonuclease 2 antibody Apurinic/apyrimidinic endonuclease 2 antibody Apurinic/apyrimidinic endonuclease like 2 antibody C430040P13Rik antibody DNA(apurinic or apyrimidinic site) lyase 2 antibody DNA apurinic or apyrimidinic site lyase 2 antibody DNA-(apurinic or apyrimidinic site) lyase 2 antibody EC 4.2.99.18 antibody OTTHUMP00000023390 antibody OTTHUMP00000061908 antibody XTH 2 antibody XTH2 antibody

Uniprot ID :

Q9UBZ4

more info or order :

Boster Immunoleader product webpage