Boster Immunoleader

antibody

Anti-GCLC Antibody

PA1492

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-GCLC Antibody

PA1492

Polyclonal

Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC(7-24aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

GCLC

Glutamate--cysteine ligase catalytic subunit

glutamate-cysteine ligase, catalytic subunit

EC 6.3.2.2 antibody Gamma ECS antibody Gamma GCS antibody Gamma glutamylcysteine synthetase antibody Gamma-ECS antibody Gamma-glutamylcysteine synthetase antibody GCL antibody GCLC antibody GCLM antibody GCS antibody GCS Heavy Chain antibody GLCL antibody GLCLC antibody GLCLR antibody Glutamate cysteine ligase catalytic subunit antibody Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD) antibody Glutamate cysteine ligase catalytic subunit antibody Glutamate cysteine ligase modifier subunit antibody Glutamate cysteine ligase regulatory protein antibody Glutamate cysteine ligase regulatory subunit antibody Glutamate--cysteine ligase catalytic subunit antibody GSC light chain antibody GSH1_HUMAN antibody

P48506