Anti-GPR2/CCR10 Antibody | Boster Immunoleader PA1491 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GPR2/CCR10 Antibody

catalog :

PA1491

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GPR2/CCR10 Antibody

Catalog Number :

PA1491

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for C-C chemokine receptor type 10(CCR10) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10(312-327aa FLGLRFRQDLRRLLRG), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+(cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.

Reference :

1. Homey, B., Alenius, H., Muller, A., Soto, H., Bowman, E. P., Yuan, W., McEvoy, L., Lauerma, A. I., Assmann, T., Bunemann, E., Lehto, M., Wolff, H., Yen, D., Marxhausen, H., To, W., Sedgwick, J., Ruzicka, T., Lehmann, P., Zlotnik, A.CCL27-CCR10 interactions regulate T cell-mediated skin inflammation.Nature Med. 8: 157-165, 2002. 2. Jarmin, D. I., Rits, M., Bota, D., Gerard, N. P., Graham, G. J., Clark-Lewis, I., Gerard, C.Cutting edge: identification of the orphan receptor G-protein-coupled receptor 2 as CCR10, a specific receptor for the chemokine ESkine.J. Immun. 164: 3460-3464, 2000. 3. Marchese, A., Docherty, J. M., Nguyen, T., Heiber, M., Cheng, R., Heng, H. H. Q., Tsui, L.-C., Shi, X., George, S. R., O'Dowd, B. F.Cloning of human genes encoding novel G protein-coupled receptors.Genomics 23: 609-618, 1994.

Gene Name :

CCR10

Protein Name :

C-C chemokine receptor type 10(C-C CKR-10/CC-CKR-10/CCR-10)

Gene Full Name :

chemokine(C-C motif) receptor 10

Synonyms :

C C chemokine receptor type 10 antibody C C CKR 10 antibody C-C chemokine receptor type 10 antibody C-C CKR-10 antibody CC chemokine receptor 10 antibody CC CKR 10 antibody CC-CKR-10 antibody CCR 10 antibody CCR-10 antibody CCR10 antibody CCR10_HUMAN antibody Chemokine(C C motif) receptor 10 antibody G protein coupled receptor 2 antibody G protein-coupled receptor 2 antibody G-protein coupled receptor 2 antibody GPR 2 antibody GPR2 antibody

Uniprot ID :

P46092

more info or order :

Boster Immunoleader product webpage