Boster Immunoleader

antibody

Anti-Aromatase Antibody

PA1489

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Aromatase Antibody

PA1489

Polyclonal

Rabbit IgG polyclonal antibody for Cytochrome P450 19A1(CYP19A1) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase(485-503aa KNMLEMIFTPRNSDRCLEH).

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

1. Chen, S., Besman, M. J., Sparkes, R. S., Zollman, S., Klisak, I., Mohandas, T., Hall, P. F., Shively, J. E.Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15.DNA 7: 27-38, 1988. 2. Harada, N., Ogawa, H., Shozu, M., Yamada, K., Suhara, K., Nishida, E., Takagi, Y.Biochemical and molecular genetic analyses on placental aromatase (P-450-AROM) deficiency.J. Biol. Chem. 267: 4781-4785, 1992. 3. Zhou, D., Pompon, D., Chen, S.Structure-function studies of human aromatase by site-directed mutagenesis: kinetic properties of mutants pro308-to-phe, tyr361-to-phe, tyr361-to-leu, and phe406-to-arg.Proc. Nat. Acad. Sci. 88: 410-414, 1991.

CYP19A1

Cytochrome P450 19A1

cytochrome P450, family 19, subfamily A, polypeptide 1

ARO 1 antibody ARO antibody ARO1 antibody Aromatase antibody CP19A_HUMAN antibody CPV antibody CPV1 antibody CYAR antibody CYP19 antibody CYP19A1 antibody CYPXIX antibody Cytochrome P-450AROM antibody Cytochrome P450 19A1 antibody Cytochrome P450 family 19 subfamily A polypeptide 1 antibody Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody Cytochrome P450, subfamily XIX(aromatization of androgens) antibody Estrogen synthase antibody Estrogen synthetase antibody Flavoprotein linked monooxygenase antibody MGC104309 antibody Microsomal monooxygenase antibody OTTHUMP00000162543 antibody OTTHUMP00000198350 antibody P 450AROM antibody P450AROM antibody

P11511