Anti-Aquaporin 3 Antibody | Boster Immunoleader PA1488 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Aquaporin 3 Antibody

catalog :

PA1488

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Aquaporin 3 Antibody

Catalog Number :

PA1488

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Aquaporin-3(AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3(278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

AQP3, Aquaporin 3, is the third member of the major intrinsic protein family from rat kidney. AQP3 is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The AQP3 gene is mapped to 9p21-p12 by fluorescence in situ hybridization. AQP3 works as water-channel. And it facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, 1 layer below the unstained stratum corneum(SC). AQP3 provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant water content and to prevent the formation of a continuous water gradient across the epidermis below the SC.

Reference :

1. Ishibashi, K., Sasaki, S., Fushimi, K., Uchida, S., Kuwahara, M., Saito, H., Furukawa, T., Nakajima, K., Yamaguchi, Y., Gojobori, T., Marumo, F.Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells.Proc. Nat. Acad. Sci. 91: 6269-6273, 1994. 2. Mulders, S. M., Olde Weghuis, D., van Boxtel, J. A. F., Geurts van Kessel, A., Echevarria, M., van Os, C. H., Deen, P. M. T.Localization of the human gene for aquaporin 3 (AQP3) to chromosome 9, region p21-p12, using fluorescent in situ hybridization.Cytogenet. Cell Genet. 72: 303-305, 1996. 3. Sougrat, R., Morand, M., Gondran, C., Barre, P., Gobin, R., Bonte, F., Dumas, M., Verbavatz, J.-M.Functional expression of AQP3 in human skin epidermis and reconstructed epidermis.J. Invest. Derm. 118: 678-685, 2002.

Gene Name :

AQP3

Protein Name :

Aquaporin-3

Gene Full Name :

aquaporin 3(Gill blood group)

Synonyms :

AQP 3 antibody AQP-3 antibody Aqp3 antibody AQP3_HUMAN antibody Aquaglyceroporin-3 antibody Aquaporin 3(GIL blood group) antibody Aquaporin 3(Gill blood group) antibody Aquaporin-3 antibody Aquaporin3 antibody GIL antibody Gill blood group antibody

Uniprot ID :

Q92482

more info or order :

Boster Immunoleader product webpage