Anti-Lipoamide Dehydrogenase Antibody | Boster Immunoleader PA1463 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Lipoamide Dehydrogenase Antibody

catalog :

PA1463

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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Boster Immunoleader product webpage

product information

Product Name :

Anti-Lipoamide Dehydrogenase Antibody

Catalog Number :

PA1463

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P; IHC-F; ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoamide Dehydrogenase(492-509aa EAFREANLAASFGKSINF), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Reference :

1. Feigenbaum, A. S., Robinson, B. H.The structure of the human dihydrolipoamide dehydrogenase gene (DLD) and its upstream elements.Genomics 17: 376-381, 1993. 2. Sakata, Y., Owada, Y., Sato, K., Kojima, K., Hisanaga, K., Shinka, T., Suzuki, Y., Aoki, Y., Satoh, J., Kondo, H., Matsubara, Y., Kure, S.Structure and expression of the glycine cleavage system in rat central nervous system.Molec. Brain Res. 94: 119-130, 2001. 3. Scherer, S. W., Otulakowski, G., Robinson, B. H., Tsui, L.-C.Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31-q32.Cytogenet. Cell Genet. 56: 176-177, 1991.

Gene Name :

DLD

Protein Name :

Dihydrolipoyl dehydrogenase, mitochondrial

Gene Full Name :

dihydrolipoamide dehydrogenase

Synonyms :

Diaphorase antibody Dihydrolipoamide dehydrogenase antibody Dihydrolipoyl dehydrogenase antibody Dihydrolipoyl dehydrogenase mitochondrial antibody dld antibody DLDH antibody DLDH_HUMAN antibody E3 antibody E3 component of pyruvate dehydrogenase antibody E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody GCSL antibody Glycine cleavage system L protein antibody Glycine cleavage system protein L antibody LAD antibody lipoamide dehydrogenase antibody Lipoamide reductase antibody Lipoyl dehydrogenase antibody mitochondrial antibody OTTHUMP00000206744 antibody OTTHUMP00000206746 antibody OTTHUMP00000206748 antibody OTTHUMP00000206749 antibody PHE 3 antibody PHE3 antibody

Uniprot ID :

P09622

more info or order :

Boster Immunoleader product webpage