Anti-CEBP Alpha Antibody | Boster Immunoleader PA1459 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CEBP Alpha Antibody

catalog :

PA1459

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-CEBP Alpha Antibody

Catalog Number :

PA1459

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for CCAAT/enhancer-binding protein alpha(CEBPA) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human CEBP Alpha(342-358aa FRQLPESSLVKAMGNCA), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

CEBPA, CCAAT/enhancer-binding protein alpha is a protein that in humans is encoded by the CEBPA gene. The CEBPA gene is intronless. Using human/hamster somatic cell hybrids containing restricted fragments of human chromosome 19, the CEBPA gene is mapped to chromosome 19q13.1, between the GPI and TGFB1 genes. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis.

Reference :

1. Helbling, D., Mueller, B. U., Timchenko, N. A., Hagemeijer, A., Jotterand, M., Meyer-Monard, S., Lister, A., Rowley, J. D., Huegli, B., Fey, M. F., Pabst, T.The leukemic fusion gene AML1-MDS1-EVI1 suppresses CEBPA in acute myeloid leukemia by activation of calreticulin.Proc. Nat. Acad. Sci. 101: 13312-13317, 2004. 2. Hendricks-Taylor, L. R., Bachinski, L. L., Siciliano, M. J., Fertitta, A., Trask, B., de Jong, P. J., Ledbetter, D. H., Darlington, G. J.The CCAAT/enhancer binding protein (C/EBP-alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP-beta) gene (CEBPB) maps to human chromosome 20q13.1.Genomics 14: 12-17, 1992. 3. Swart, G. W. M., van Groningen, J. J. M., van Ruissen, F., Bergers, M., Schalkwijk, J.Transcription factor C/EBP-alpha: novel sites of expression and cloning of the human gene.Biol. Chem. 378: 373-379, 1997.

Gene Name :

CEBPA

Protein Name :

CCAAT/enhancer-binding protein alpha(C/EBP alpha)

Gene Full Name :

CCAAT/enhancer binding protein(C/EBP), alpha

Synonyms :

Apoptotic cysteine protease antibody Apoptotic protease Mch 5 antibody C/EBP alpha antibody C/ebpalpha antibody CAP4 antibody Caspase 8 precursor antibody CBF-A antibody CCAAT Enhancer Binding Protein alpha antibody CCAAT/enhancer binding protein(C/EBP), alpha antibody CCAAT/enhancer-binding protein alpha antibody CEBP antibody CEBP A antibody CEBP alpha antibody Cebpa antibody CEBPA_HUMAN antibody FADD homologous ICE/CED 3 like protease antibody FADD like ICE antibody FLICE antibody ICE like apoptotic protease 5 antibody ICE8 antibody MACH antibody MCH5 antibody MORT1 associated CED 3 homolog antibody

Uniprot ID :

P49715

more info or order :

Boster Immunoleader product webpage