Boster Immunoleader

antibody

Anti-KRAS Antibody

PA1457

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-KRAS Antibody

PA1457

Polyclonal

Rabbit IgG polyclonal antibody for GTPase KRas(KRAS) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human KRAS(164-178aa/b RKHKEKMSKDGKKKK), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

K-ras, V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, is a protein that in humans is encoded by the KRAS gene. Like other members of the Ras family, the K-ras protein is a GTPase and is an early player in many signal transduction pathways. K-ras is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. The K-ras gene spans 38 kb and contains 4 exons. By in situ hybridization, the K-ras gene is mapped to chromosome 12p12.1-p11.1. K-ras acts as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. K-ras binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP

1. Popescu, N. C., Amsbaugh, S. C., DiPaolo, J. A., Tronick, S. R., Aaronson, S. A., Swan, D. C.Chromosomal localization of three human ras genes by in situ molecular hybridization.Somat. Cell Molec. Genet. 11: 149-155, 1985. 2. Porta, M., Malats, N., Jariod, M., Grimalt, J. O., Rifa, J., Carrato, A., Guarner, L., Salas, A., Santiago-Silva, M., Corominas, J. M., Andreu, M., Real, F. X.Serum concentrations of organochlorine compounds and K-ras mutations in exocrine pancreatic cancer. 3. Weinberg, R. A.Fewer and fewer oncogenes.Cell 30: 3-4, 1982.

KRAS

GTPase Kras

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

c Kirsten ras protein antibody c-K-ras antibody c-Ki-ras antibody Cellular c Ki ras2 proto oncogene antibody cK Ras antibody GTPase KRas antibody K RAS p21 protein antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody K-Ras 2 antibody KI RAS antibody Ki-Ras antibody KIRSTEN MURINE SARCOMA VIRUS 2 antibody Kirsten rat sarcoma 2 viral(v Ki ras2) oncogene homolog antibody KRAS antibody KRAS1 antibody KRAS2 antibody N-terminally processed antibody NS antibody NS3 antibody Oncogene KRAS2 antibody p21ras antibody PR310 cK ras oncogene antibody RASK_HUMAN antibody RASK2 antibody Transforming protein p21 antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody

P01116