Boster Immunoleader

antibody

Anti-Cyclophilin A Antibody

PA1447

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Cyclophilin A Antibody

PA1447

Polyclonal

Rabbit IgG polyclonal antibody for Peptidyl-prolyl cis-trans isomerase A(PPIA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclophilin A(54-71aa HRIIPGFMCQGCDFTRHN), different from the related mouse and rat sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Cyclophilin A(PPIA),Peptidylprolyl isomerase A,is an enzyme that in humans is encoded by the PPIA gene.Using chromosome 7 and chromosome 10 deletion hybrid panels, the PPIA coding gene is localized to 7p13-p11.2. This gene encodes a member of the peptidyl-prolyl cis-trans isomerase(PPIase) family. Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and are believed to be involved in protein folding and/or intracellular protein transport. Cyclophilin A binds to the Gag protein of human immunodeficiency virus type 1(HIV-1). Cyclophilin A may have an essential function in HIV-1 replication.may have an essential function in HIV-1 replication.

1. Luban, J., Bossolt, K. L., Franke, E. K., Kalpana, G. V., Goff, S. P.Human immunodeficiency virus type 1 gag protein binds to Cyclophilin A and B.Cell 73: 1067-1078, 1993. 2. Manel, N., Hogstad, B., Wang, Y., Levy, D. E., Unutmaz, D., Littman, D. R.A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells.Nature 467: 214-217, 2010. Note: Erratum: Nature 470: 424 only, 2011. 3. Willenbrink, W., Halaschek, J., Schuffenhauer, S., Kunz, J., Steinkasserer, A. Cyclophilin A, the major intracellular receptor for the immunosuppressant cyclosporin A, maps to chromosome 7p11.2-p13: four pseudogenes map to chromosomes 3, 10, 14, and 18.Genomics 28: 101-104, 1995.

PPIA

Peptidyl-prolyl cis-trans isomerase A(PPIase A)

peptidylprolyl isomerase A(cyclophilin A)

Cyclophilin A Cyclosporin A-binding protein Rotamase A

P62937