Anti-Chk1 Antibody | Boster Immunoleader PA1445 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Chk1 Antibody

catalog :

PA1445

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Chk1 Antibody

Catalog Number :

PA1445

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Chk1(CHEK1) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CChk1(69-87aa KFYGHRREGNIQYLFLEYC), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CHEK1, Cell cycle checkpoint kinase, is an enzyme that in humans is encoded by the CHEK1 gene. By fluorescence in situ hybridization,the human CHEK1 gene is mapped to 11q24, near the ATM gene at 11q23. CHEK1is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. CHEK1 acts to integrate signals from ATM and ATR, and is involved in monitoring meiotic recombination, a process that involves programmed DNA breaks.

Reference :

1. Flaggs, G., Plug, A. W., Dunks, K. M., Mundt, K. E., Ford, J. C., Quiggle, M. R. E., Taylor, E. M., Westphal, C. H., Ashley, T., Hoekstra, M. F., Carr, A. M. Atm-dependent interactions of a mammalian Chk1 homolog with meiotic chromosomes. Curr. Biol. 7: 977-986, 1997. 2. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., Elledge, S. J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-1501, 1997. 3. Zhang, Y.-W., Otterness, D. M., Chiang, G. G., Xie, W., Liu, Y.-C., Mercurio, F., Abraham, R. T. Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway. Molec. Cell 19: 607-618, 2005.

Gene Name :

CHEK1

Protein Name :

Serine/threonine-protein kinase Chk1

Gene Full Name :

checkpoint kinase 1

Synonyms :

C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog(S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog(S. pombe) antibody CHK1_HUMAN antibody EC 2.7.11.1 antibody rad27 antibody Serine/threonine protein kinase Chk1 antibody Serine/threonine-protein kinase CHK1 antibody STT3, subunit of the oligosaccharyltransferase complex, homolog A(S. cerevisiae) antibody

Uniprot ID :

O14757

more info or order :

Boster Immunoleader product webpage