Boster Immunoleader

antibody

Anti-Cdc34 Antibody

PA1444

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Cdc34 Antibody

PA1444

Polyclonal

Rabbit IgG polyclonal antibody for Ubiquitin-conjugating enzyme E2 R1(CDC34) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human Cdc34(148-163aa YRKWKESKGKDREYTD), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CDC34, Cell division cycle 34, Ubiquitin-conjugating enzyme, is a protein that in humans is encoded by the CDC34 gene. The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. By in situ hybridization that the CDC34 gene is located in the far telomeric region of 19p13.3, in a region of syntenic homology between human 19p and mouse 11.Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.

1. Pierce, N. W., Kleiger, G., Shan, S., Deshaies, R. J. Detection of sequential polyubiquitylation on a millisecond timescale. 2. Plon, S. E., Leppig, K. A., Do, H.-N., Groudine, M. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. 3. Semplici, F., Meggio, F., Pinna, L. A., Oliviero, S. CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation.

CDC34

Ubiquitin-conjugating enzyme E2 R1

cell division cycle 34 homolog(S. cerevisiae)

Cdc 34 antibody Cdc34 antibody Cell division cycle 34 antibody Cell division cycle 34 homolog(S. cerevisiae) antibody Cell division cycle 34 homolog antibody E2 CDC34 antibody UB2R1_HUMAN antibody UBC 3 antibody UBC3 antibody UBCH3 antibody UBE2 R1 antibody UBE2R1 antibody Ubiquitin carrier protein antibody Ubiquitin conjugating enzyme Cdc34 antibody Ubiquitin conjugating enzyme E2 32 kDa complementing antibody Ubiquitin protein ligase antibody Ubiquitin protein ligase R1 antibody Ubiquitin-conjugating enzyme E2 R1 antibody Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody Ubiquitin-conjugating enzyme E2-CDC34 antibody Ubiquitin-protein ligase R1 antibody

P49427