Anti-Caspase-1(P20) Antibody | Boster Immunoleader PA1440 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Caspase-1(P20) Antibody

catalog :

PA1440

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, et al. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. Int J Mol Med. 2015;35:1179-88 pubmed publisher
Kouadir M, Yang L, Tan R, Shi F, Lu Y, Zhang S, et al. CD36 participates in PrP(106-126)-induced activation of microglia. PLoS ONE. 2012;7:e30756 pubmed publisher
Li H, Deng C, Chen B, Zhang S, Liang Y, Luo X. Total saponins of Panax notoginseng modulate the expression of caspases and attenuate apoptosis in rats following focal cerebral ischemia-reperfusion. J Ethnopharmacol. 2009;121:412-8 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Caspase-1(P20) Antibody

Catalog Number :

PA1440

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Caspase-1(CASP1) detection. Tested with WB, IHC-P in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human CASP1(135-152aa CSLEEAQRIWKQKSAEIY).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

Reference :

1. Cerretti, D. P., Hollingsworth, L. T., Kozlosky, C. J., Valentine, M. B., Shapiro, D. N., Morris, S. W., Nelson, N.Molecular characterization of the gene for human interleukin-1-beta converting enzyme (IL1BC).Genomics 20: 468-473, 1994. 2. Thornberry, N. A., Bull, H. G., Calaycay, J. R., Chapman, K. T., Howard, A. D., Kostura, M. J., Miller, D. K., Molineaux, S. M., Weidner, J. R., Aunins, J., Elliston, K. O., Ayala, J. M., Casano, F. J., Chin, J., Ding, G. J.-F., Egger, L. A., Gaffney, E. P., Limjuco, G., Palyha, O. C., Raju, S. M., Rolando, A. M., Salley, J. P., Yamin, T.-T., Lee, T. D., Shively, J. E., MacCross, M., Mumford, R. A., Schmidt, J. A., Tocci, M. J.A novel heterodimeric cysteine protease is required for interleukin-1-beta processing in monocytes.Nature 356: 768-774, 1992.

Gene Name :

CASP1

Protein Name :

Caspase-1(CASP-1)

Gene Full Name :

caspase 1, apoptosis-related cysteine peptidase

Synonyms :

CASP 1 antibody CASP-1 antibody CASP1 antibody Caspase 1 apoptosis related cysteine peptidase antibody Caspase 1 apoptosis related cysteine protease antibody Caspase-1 subunit p20 antibody Caspase1 antibody ICE antibody IL 1 beta converting enzyme antibody IL 1BC antibody IL-1 beta-converting enzyme antibody IL-1BC antibody IL1 beta converting enzyme antibody IL1B convertase antibody IL1BC antibody IL1BCE antibody Interleukin 1 beta convertase antibody Interleukin 1 beta convertase precursor antibody Interleukin 1 beta converting enzyme antibody Interleukin 1B converting enzyme antibody Interleukin-1 beta convertase antibody Interleukin-1 beta-converting enzyme antibody P45 antibody

Uniprot ID :

P29466

more info or order :

Boster Immunoleader product webpage