Boster Immunoleader

antibody

Anti-BAK Antibody

PA1437

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-BAK Antibody

PA1437

Polyclonal

Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human BAK1(74-95aa QVGRQLALIGDDINRRYDSEFQ), different from the related mouse and rat sequences by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene.The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons.By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6.This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

1. Chittenden, T., Harrington, E. A., O'Connor, R., Flemington, C., Lutz, R. J., Evan, G. I., Guild, B. C.Induction of apoptosis by the Bcl-2 homologue Bak.Nature 374: 733-736, 1995. 2. Herberg, J. A., Phillips, S., Beck, S., Jones, T., Sheer, D., Wu, J. J., Prochazka, V., Barr, P. J., Kiefer, M. C., Trowsdale, J.Genomic structure and domain organisation of the human Bak gene.Gene 211: 87-94, 1998. 3. Kiefer, M. C., Brauer, M. J., Powers, V. C., Wu, J. J., Umansky, S. R., Tomei, L. D., Barr, P. J.Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.Nature 374: 736-739, 1995.

BAK1

Bcl-2 homologous antagonist/killer

BCL2-antagonist/killer 1

Apoptosis Regulator Bak antibody BAK antibody BAK like antibody Bak NT antibody BAK_HUMAN antibody BAK1 antibody Bcl 2 homologous antagonist/killer antibody Bcl 2 like 7 protein antibody Bcl-2 homologous antagonist/killer antibody Bcl-2-like protein 7 antibody BCL2 antagonist/killer 1 antibody Bcl2 like 7 Protein antibody Bcl2-L-7 antibody BCL2L7 antibody CDN1 antibody Cell death inhibitor 1 antibody MGC117255 antibody MGC3887 antibody NBak antibody Pro apoptotic protein BAK antibody

Q16611