Boster Immunoleader

antibody

Anti-Osteopontin Antibody

PA1431

100 ug/vial

200 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Osteopontin Antibody

PA1431

Polyclonal

Rabbit IgG polyclonal antibody for Osteopontin(SPP1) detection. Tested with WB, IHC-P in Human.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin(28-47aa EEKQLYNKYPDAVATWLNPD), different from the related mouse sequence by six amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

1. Beck, G. R., Jr.; Zerler, B.; Moran, E. Phosphate is a specific signal for induction of osteopontin gene expression. Proc. Nat. Acad. Sci. 97: 8352-8357, 2000. 2. Kohri, K.; Suzuki, Y.; Yoshida, K.; Yamamoto, K.; Amasaki, N.; Yamate, T.; Umekawa, T.; Iguchi, M.; Sinohara, H.; Kurita, T. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. Biochem. Biophys. Res. Commun. 184: 859-864, 1992. 3. Fisher, L. W.; McBride, O. W.; Termine, J. D.; Young, M. F. Human bone sialoprotein: deduced protein sequence and chromosomal localization. J. Biol. Chem. 265: 2347-2351, 1990.

SPP1

Osteopontin

secreted phosphoprotein 1

BNSP antibody Bone sialoprotein 1 antibody BSP I antibody BSPI antibody Early T lymphocyte activation 1 antibody ETA 1 antibody ETA1 antibody MGC110940 antibody Nephropontin antibody OPN antibody Osteopontin antibody osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody OSTP_HUMAN antibody PSEC0156 antibody secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody Secreted phosphoprotein 1 antibody SPP 1 antibody SPP-1 antibody SPP1 antibody SPP1/CALPHA1 fusion antibody Urinary stone protein antibody Uropontin antibody

P10451