Anti-Ubiquitin Antibody | Boster Immunoleader PA1420 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Ubiquitin Antibody

catalog :

PA1420

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Ubiquitin Antibody

Catalog Number :

PA1420

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Polyubiquitin-B(UBB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin(29-45aa KIQDKEGIPPDQQRLIF), identical to the rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Ubiquitin(originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues(ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Reference :

he Nobel Prize in Chemistry 2004". Nobelprize.org. 2. Kashuba, V. I., Gizatullin, R. Z., Protopopov, A. I., Allikmets, R., Korolev, S., Li, J., Boldog, F., Tory, K., Zabarovska, V., Marcsek, Z., Sumegi, J., Klein, G., Zabarovsky, E. R., Kisselev, L.NotI linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukemia and deleted in solid tumors.FEBS Lett.419: 181-185, 1997.

Gene Name :

UBB

Protein Name :

Polyubiquitin-B

Gene Full Name :

ubiquitin B

Synonyms :

FLJ25987 antibody MGC8385 antibody Polyubiquitin B antibody RPS 27A antibody RPS27A antibody UBA 52 antibody UBA 80 antibody UBA52 antibody UBA80 antibody UBB antibody UBB_HUMAN antibody UBC antibody UBCEP 1 antibody UBCEP 2 antibody UBCEP1 antibody UBCEP2 antibody Ubiquitin antibody ubiquitin B antibody

Uniprot ID :

P0CG47

more info or order :

Boster Immunoleader product webpage