Boster Immunoleader

antibody

Anti-Pleiotrophin Antibody

PA1414

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Pleiotrophin Antibody

PA1414

Polyclonal

Rabbit IgG polyclonal antibody for Pleiotrophin(PTN) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Pleiotrophin(150-168aa QAESKKKKKEGKKQEKMLD), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Pleiotrophin(PTN) also known as heparin-binding brain mitogen(HBBM) or heparin-binding growth factor 8(HBGF-8) or neurite growth-promoting factor 1(NEGF1) or heparin affinity regulatory peptide(HARP) or heparin binding growth associated molecule(HB-GAM) is a protein that in humans is encoded by the PTN gene. PTN is the first member of a family of developmentally regulated cytokines. The PTN gene is mapped to 7q33-q34. A mutant PTN that contained only the first N-terminal 40 amino acids was a dominant negative. Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin binds to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection.

1. Entrez Gene: PTN pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)". 2. Vanderwinden JM, Mailleux P, Schiffmann SN, Vanderhaeghen JJ (1992). "Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues". Anat. Embryol. 186 (4): 387–406. 3. Said EA, Courty J, Svab J, Delbé J, Krust B, Hovanessian AG (September 2005). "Pleiotrophin inhibits HIV infection by binding the cell surface-expressed nucleolin". FEBS J. 272 (18): 4646–59.

PTN

Pleiotrophin(PTN)

pleiotrophin

HARP antibody HB-GAM antibody HBBM antibody HBGAM antibody HBGF-8 antibody HBGF8 antibody HBNF antibody HBNF-1 antibody HBNF1 antibody Heparin binding growth associated molecule antibody Heparin binding growth factor 8 antibody Heparin binding neurite outgrowth promoting factor 1 antibody Heparin-binding brain mitogen antibody Heparin-binding growth factor 8 antibody Heparin-binding growth-associated molecule antibody Heparin-binding neurite outgrowth-promoting factor 1 antibody NEGF1 antibody Neurite growth promoting factor 1 antibody OSF-1 antibody OSF1 antibody Osteoblast-specific factor 1 antibody Pleiotrophin antibody PTN antibody PTN_HUMAN antibody

P21246