Anti-MGMT Antibody | Boster Immunoleader PA1408 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-MGMT Antibody

catalog :

PA1408

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-MGMT Antibody

Catalog Number :

PA1408

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Methylated-DNA-protein-cysteine methytransferase(MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al.(1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al.(1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis.

Reference :

1. Tano K, Shiota S, Collier J, Foote RS, Mitra S (January 1990). "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine". Proc. Natl. Acad. Sci. U.S.A. 87 (2): 686–90. 2. Natarajan AT, Vermeulen S, Darroudi F, Valentine MB, Brent TP, Mitra S, Tano K (January 1992). "Chromosomal localization of human O6-methylguanine-DNA methyltransferase (MGMT) gene by in situ hybridization". Mutagenesis 7 (1): 83–5. 3. Gardner, E., Rydberg, B., Karran, P., Ponder, B. A. J.Localization of the human O(6)-methylguanine-DNA methyltransferase gene to chromosome 10q24.33-qter.Genomics11: 475-476, 1991.

Gene Name :

MGMT

Protein Name :

Methylated-DNA--protein-cysteine methyltransferase

Gene Full Name :

O-6-methylguanine-DNA methyltransferase

Synonyms :

6 O methylguanine DNA methyltransferase antibody Agat antibody AGT antibody AI267024 antibody EC 2.1.1.63 antibody Methylated DNA protein cysteine methyltransferase antibody Methylguanine DNA methyltransferase antibody MGC107020 antibody MGMT antibody MGMT: O-6 methylguanine DNA methyltransferase antibody O 6 methylguanine DNA alkyltransferase antibody O 6 methylguanine DNA methyltransferase antibody O-6 methylguanine DNA alkyltransferase antibody O-6-methylguanine-DNA methyltransferase antibody O6 methylguanine DNA methyltransferase antibody

Uniprot ID :

P16455

more info or order :

Boster Immunoleader product webpage