Anti-GADD45A Antibody | Boster Immunoleader PA1402 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GADD45A Antibody

catalog :

PA1402

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GADD45A Antibody

Catalog Number :

PA1402

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A(129-143aa NPHSSQWKDPALSQL), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Growth arrest and DNA-damage-inducible protein GADD45 alpha(GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al.(1991) demonstrated that the GADD45 gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.

Reference :

1. Papathanasiou MA, Kerr NC, Robbins JH, McBride OW, Alamo I Jr, Barrett SF, Hickson ID, Fornace AJ Jr (Mar 1991). "Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C". Mol Cell Biol 11 (2): 1009–16. 2. Barreto, G., Schafer, A. Marhold, J., Stach, D., Swaminathan, S. K., Handa, V., Doderlein, G., Maltry, N., Wu, W., Lyko, F., Niehrs, C.Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.Nature445: 671-675, 2007.

Gene Name :

GADD45A

Protein Name :

Growth arrest and DNA damage-inducible protein GADD45 alpha

Gene Full Name :

growth arrest and DNA-damage-inducible, alpha

Synonyms :

DDIT 1 antibody DDIT-1 antibody DDIT1 antibody DNA damage inducible transcript 1 antibody DNA damage-inducible transcript 1 protein antibody GA45A_HUMAN antibody GADD45 antibody GADD45A antibody Growth arrest and DNA damage inducible 45 alpha antibody Growth arrest and DNA damage inducible alpha antibody Growth arrest and DNA damage-inducible protein GADD45 alpha antibody

Uniprot ID :

P24522

more info or order :

Boster Immunoleader product webpage