Anti-EGF Antibody | Boster Immunoleader PA1398 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-EGF Antibody

catalog :

PA1398

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Meng H, Chen L, Ye Z, Wang S, Zhao X. The effect of a self-assembling peptide nanofiber scaffold (peptide) when used as a wound dressing for the treatment of deep second degree burns in rats. J Biomed Mater Res B Appl Biomater. 2009;89:379-91 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-EGF Antibody

Catalog Number :

PA1398

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, IHC-P in Mouse.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Reference :

1. Carpenter G, Cohen S (May 1990). "Epidermal growth factor". The Journal of Biological Chemistry 265 (14): 7709–12. PMID 2186024. 2. Herbst RS (2004). "Review of epidermal growth factor receptor biology". International Journal of Radiation Oncology, Biology, Physics 59 (2 Suppl): 21–6. doi:10.1016/j.ijrobp.2003.11.041. PMID 15142631. 3. Brissenden, J. E., Ullrich, A., Francke, U.Chromosomal mapping of loci for insulin-like growth factors I and II and for epidermal growth factor in man.Am. J. Hum. Genet.36: 133S-only, 1984.

Gene Name :

EGF

Protein Name :

Pro-epidermal growth factor(EGF)

Gene Full Name :

epidermal growth factor

Synonyms :

Beta urogastrone antibody EGF antibody Egf antibody EGF_HUMAN antibody Epidermal Growth Factor antibody Epidermal Growth Factor antibody HOMG4 antibody OTTHUMP00000219721 antibody OTTHUMP00000219722 antibody Pro epidermal growth factor antibody URG antibody Urogastrone antibody

Uniprot ID :

P01132

more info or order :

Boster Immunoleader product webpage