Boster Immunoleader

antibody

Anti-ABCB11 Antibody

PA1393

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse

western blot, immunohistochemistry - paraffin section

Anti-ABCB11 Antibody

PA1393

Polyclonal

Rabbit IgG polyclonal antibody for Bile salt export pump(ABCB11) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11(1299-1313aa THEELMAQKGAYYKL), different from the related rat sequence by one amino acid, and from the mouse sequence by three amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Bile Salt Export Pump(BSEP) is a protein which in humans is encoded by the ABCB11 gene. It is a member of the superfamily of ATP-binding cassette(ABC) transporters, also known as ABCB11. It is mapped to chromosome 2q24. The BSEP protein is mainly expressed in the liver. ABCB11 is a gene associated with progressive familial intrahepatic cholestasis type 2(PFIC2) which caused by mutations in the ABCB11 gene will increases the risk of hepatocellular carcinoma in early life.

1. Strautnieks SS, Bull LN, Knisely AS, Kocoshis SA, Dahl N, Arnell H, Sokal E, Dahan K, Childs S, Ling V, Tanner MS, Kagalwalla AF, Németh A, Pawlowska J, Baker A, Mieli-Vergani G, Freimer NB, Gardiner RM, Thompson RJ (November 1998). "A gene encoding a liver-specific ABC transporter is mutated in progressive familial intrahepatic cholestasis". Nat. Genet. 20 (3): 233–8. 2. Strautnieks, S., Kagalwalla, A. F., Tanner, M. S., Knisely, A. S., Bull, L. N., Freimer, N. B., Kocoshis, S. A., Gardiner, R. M., Thompson, R. J.Identification of a second locus for progressive familial intrahepatic cholestasis on chromosome 2q24.Medizinische Genetik9: 16-only, 1997. 3. Noe J, Kullak-Ublick GA, Jochum W, Stieger B, Kerb R, Haberl M, Müllhaupt B, Meier PJ, Pauli-Magnus C (September 2005). "Impaired expression and function of the bile salt export pump due to three novel ABCB11 mutations in intrahepatic cholestasis". J. Hepatol. 43 (3): 536–43. 4. Knisely AS, Strautnieks SS, Meier Y, Stieger B, Byrne JA, Portmann BC, Bull LN, Pawlikowska L, Bilezikçi B, Ozçay F, László A, Tiszlavicz L, Moore L, Raftos J, Arnell H, Fischler B, Németh A, Papadogiannakis N, Cielecka-Kuszyk J, Jankowska I, Pawłowska J, Melín-Aldana H, Emerick KM, Whitington PF, Mieli-Vergani G, Thompson RJ (August 2006). "Hepatocellular carcinoma in ten children under five years of age with bile salt export pump deficiency". Hepatology 44 (2): 478–86.

ABCB11

Bile salt export pump

ATP-binding cassette, sub-family B(MDR/TAP), member 11

ABC member 16, MDR/TAP subfamily antibody ABCB1 antibody Abcb11 antibody ABCBB_HUMAN antibody ATP binding cassette sub family B(MDR/TAP) member 11 antibody ATP binding cassette sub family B member 11 antibody ATP-binding cassette sub-family B member 11 antibody Bile salt export pump antibody BRIC2 antibody Bsep antibody PGY4 antibody progressive familial intrahepatic cholestasis 2 antibody Sister of P glycoprotein antibody Spgp antibody

O95342