Anti-Bag1 Antibody | Boster Immunoleader PA1392 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Bag1 Antibody

catalog :

PA1392

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Bag1 Antibody

Catalog Number :

PA1392

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 1(BAG1) detection. Tested with WB, IHC-P, ICC in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Bag1(322-344aa DTVEQNICQETERLQSTNFALAE), different from the related mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

BAG family molecular chaperone regulator 1(BAG1) is a protein that in humans is encoded by the BAG1 gene. Human BAG1 is mapped to chromosome 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death. The Bag1 protein is rich in glutamic acid residues. Its deduced 274-amino acid protein has a calculated molecular mass of 31 KD. Being the BCL-2-associated athanogene, Bag1 enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.

Reference :

1. Takayama S, Sato T, Krajewski S, Kochel K, Irie S, Millan JA, Reed JC (Mar 1995). "Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity". Cell 80 (2): 279–84. 2. Takayama, S., Kochel, K., Irie, S., Inazawa, J., Abe, T., Sato, T., Druck, T., Huebner, K., Reed, J. C.Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.Genomics35: 494-498, 1996. 3. "Entrez Gene: BAG1 BCL2-associated athanogene".

Gene Name :

BAG1

Protein Name :

BAG family molecular chaperone regulator 1(BAG-1)

Gene Full Name :

BCL2-associated athanogene

Synonyms :

Bcl-2-associated athanogene 1

Uniprot ID :

Q99933

more info or order :

Boster Immunoleader product webpage