Boster Immunoleader

antibody

Anti-MEK3 Antibody

PA1377

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-MEK3 Antibody

PA1377

Polyclonal

Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 3(MAP2K3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3(320-334aa MEHPFFTLHKTKKTD), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al.(1997) localized the MAP2K3 gene to 17q11.2.

1. Rampoldi L, Zimbello R, Bortoluzzi S, Tiso N, Valle G, Lanfranchi G, Danieli GA (Mar 1998). "Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5". Cytogenet Cell Genet 78 (3-4): 301–3.

MAP2K3

Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3)

mitogen-activated protein kinase kinase 3

AW212142 antibody Dual specificity mitogen activated protein kinase kinase 3 antibody Dual specificity mitogen-activated protein kinase kinase 3 antibody ERK kinase 3 antibody MAP kinase kinase 3 antibody MAP2K 3 antibody map2k3 antibody MAPK ERK kinase 3 antibody MAPK kinase 3 antibody MAPK/ERK kinase 3 antibody MAPKK 3 antibody MAPKK3 antibody MEK 3 antibody MEK3 antibody Mitogen activated protein kinase kinase 3 antibody MKK 3 antibody MKK3 antibody mMKK 3b antibody mMKK3b antibody MP2K3_HUMAN antibody MPK 3 antibody PRKMK 3 antibody PRKMK3 antibody Protein kinase mitogen activated kinase 3 antibody protein kinase, mitogen-activated, kinase 3 antibody SAPK kinase 2 antibody SAPKK 2 antibody SAPKK2 antibody SKK2 antibody Stress activated protein kinase kinase 2 antibody zMKK 3 antibody

P46734