Anti-Connexin 32/GJB1 Antibody | Boster Immunoleader PA1367 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Connexin 32/GJB1 Antibody

catalog :

PA1367

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Connexin 32/GJB1 Antibody

Catalog Number :

PA1367

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Gap junction beta-1 protein(GJB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1(215-231aa RACARRAQRRSNPPSRK), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.

Reference :

1. Corcos IA, Lafreniere RG, Begy CR, Loch-Caruso R, Willard HF, Glover TW (Jul 1992). "Refined localization of human connexin32 gene locus, GJB1, to Xq13.1". Genomics 13 (2): 479–80. 2. Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76.

Gene Name :

GJB1

Protein Name :

Gap junction beta-1 protein

Gene Full Name :

gap junction protein, beta 1, 32kDa

Synonyms :

Charcot Marie Tooth neuropathy X linked antibody CMTX 1 antibody CMTX antibody CMTX1 antibody Connexin-32 antibody Connexin32 antibody CX 32 antibody CX32 antibody CXB1_HUMAN antibody GAP junction 28 kDa liver protein antibody Gap junction beta 1 protein antibody Gap junction beta-1 protein antibody Gap junction protein beta 1 32kD antibody Gap junction protein beta 1 antibody GJB 1 antibody GJB1 antibody

Uniprot ID :

P08034

more info or order :

Boster Immunoleader product webpage