Anti-CD80 Antibody | Boster Immunoleader PA1365-1 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD80 Antibody

catalog :

PA1365-1

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat

application :

western blot

publications citing this reagent :

DU H, Dong L, Zhao B, Fu J, Wang Q, Chen F, et al. Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice. Acta Pharmacol Sin. 2012;33:1047-54 pubmed publisher
Chen P, Chen R, Yang Y, Yu Y, Xie Y, Zou Y, et al. Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium. Int Immunopharmacol. 2009;9:1304-12 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CD80 Antibody

Catalog Number :

PA1365-1

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat

Notes :

WB: The detection limit for CD80 is approximately 1.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Reference :

1. Peach, R J; Bajorath J, Naemura J, Leytze G, Greene J, Aruffo A, Linsley P S (Sep. 1995). "Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28". J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7. 2. Stamper, C C; Zhang Y, Tobin J F, Erbe D V, Ikemizu S, Davis S J, Stahl M L, Seehra J, Somers W S, Mosyak L (Mar. 2001). "Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses". Nature (England) 410 (6828): 608–11.

Gene Name :

CD80

Protein Name :

T-lymphocyte activation antigen CD80

Gene Full Name :

CD80 molecule

Synonyms :

Activation B7 1 antigen antibody Activation B7-1 antigen antibody Activation B71 antigen antibody B lymphocyte activation antigen B7 antibody B7 antibody B7-1 antibody B7-1 antigen antibody BB1 antibody CD 80 antibody CD28 antigen ligand 1 antibody CD28 antigen ligand 1 B7 1 antigen antibody CD28 antigen ligand 1 B71 antigen antibody CD28LG antibody CD28LG1 antibody CD80 antibody CD80 antigen antibody CD80 antigen precursor antibody CD80 molecule antibody CD80_HUMAN antibody Costimulatory factor CD80 antibody CTLA 4 counter receptor B7.1 antibody CTLA-4 counter-receptor B7.1 antibody LAB 7 antibody LAB7 antibody T lymphocyte activation antigen CD80 antibody T-lymphocyte activation antigen CD80 antibody

Uniprot ID :

O55202

more info or order :

Boster Immunoleader product webpage