Anti-Calpain 1 Antibody | Boster Immunoleader PA1364 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Calpain 1 Antibody

catalog :

PA1364

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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product information

Product Name :

Anti-Calpain 1 Antibody

Catalog Number :

PA1364

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,IHC-F,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Frozen Section) :

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC.

Background :

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS; 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

Reference :

1. Aoki K, Imajoh S, Ohno S, Emori Y, Koike M, Kosaki G, Suzuki K (Oct 1986). "Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence".

Gene Name :

CAPN1

Protein Name :

Calpain-1 catalytic subunit

Gene Full Name :

calpain 1,(mu/I) large subunit

Synonyms :

Ca2 activated neutral protease antibody Calcium activated neutral proteinase 1 antibody Calcium activated neutral proteinase antibody Calcium activated neutral proteinase small subunit antibody Calcium dependent protease small subunit 1 antibody Calcium dependent protease small subunit antibody Calcium-activated neutral proteinase 1 antibody Calpain 1(mu/I) large subunit antibody Calpain 1 antibody Calpain 1 catalytic subunit antibody Calpain 1 large subunit antibody Calpain Large Polypeptide L1 antibody Calpain Large Polypeptide L1 antibody Calpain mu type antibody Calpain mu-type antibody Calpain regulatory subunit antibody Calpain small subunit 1 antibody Calpain-1 catalytic subunit antibody Calpain-1 large subunit antibody Calpain1 antibody CAN1_HUMAN antibody CANP 1 antibody CANP antibody CANP small subunit antibody CANPL 1 antibody CANPL1 antibody CAPN 1 antibody CAPN1 antibody Cell proliferation inducing protein 30 antibody Cell proliferation-inducing gene 30 protein antibody Micromolar Calpain antibody Micromolar-calpain antibody Mu Calpain antibody muCANP antibody muCL antibody

Uniprot ID :

P07384

more info or order :

Boster Immunoleader product webpage