Anti-ERK1 Antibody | Boster Immunoleader PA1343 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ERK1 Antibody

catalog :

PA1343

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

publications citing this reagent :

Wang Y, Tian F, Liu H, Zhang W, Li J, Xiao B, et al. Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients. J Neuroimmunol. 2013;257:67-75 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ERK1 Antibody

Catalog Number :

PA1343

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 3(MAPK3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human ERK1(287-301aa KTKVAWAKLFPKSDS), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.

Reference :

1. Garcia F, Zalba G, Paez G, Encio I, de Miguel C (Apr 1999). "Molecular cloning and characterization of the human p44 mitogen-activated protein kinase gene". Genomics 50 (1): 69–78. 2. "Entrez Gene: MAPK3 mitogen-activated protein kinase 3". 3. Charest, D. L., Mordret, G., Harder, K. W., Jirik, F., Pelech, S. L. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1. Molec. Cell. Biol. 13: 4679-4690, 1993.

Gene Name :

MAPK3

Protein Name :

Mitogen-activated protein kinase 3(MAP kinase 3/MAPK 3)

Gene Full Name :

mitogen-activated protein kinase 3

Synonyms :

ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody

Uniprot ID :

P27361

more info or order :

Boster Immunoleader product webpage