Anti-Hsp90 alpha Antibody | Boster Immunoleader PA1339 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hsp90 alpha Antibody

catalog :

PA1339

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Hsp90 alpha Antibody

Catalog Number :

PA1339

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse; Predicted Species: Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp90 alpha(676-693aa/(2) FSLEDPQTHANRIYRMIK), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Hsp90(heat shock protein 90) is a molecular chaperone and is one of the most abundant proteins expressed in cells. It is a member of the heat shock protein family which is upregulated in response to stress. Hsp90 is found in bacteria and all branches of eukarya, but it is apparently absent in archaea. Cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions. The function of Hsp90 includes assisting in protein folding, cell signaling, and tumor repression. This protein was first isolated by extracting proteins from stressed cells. These cells were stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature. Researchers later realized that Hsp90 has other essential functions in unstressed cells.

Reference :

1. Csermely P, Schnaider T, Soti C, Prohászka Z, Nardai G (August 1998). "The 90-kDa molecular chaperone family: structure, function, and clinical applications. A comprehensive review". Pharmacol. Ther. 79 (2): 129–68. 2. Chen B, Zhong D, Monteiro A (2006). "Comparative genomics and evolution of the Hsp90 family of genes across all kingdoms of organisms". BMC Genomics 7: 156. 3. Thomas JG, Baneyx F (October 1998). "Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in thermal stress management: comparison with ClpA, ClpB, and HtpG In vivo". J. Bacteriol. 180 (19): 5165–72. 4. Prodromou C, Panaretou B, Chohan S, Siligardi G, O'Brien R, Ladbury JE, Roe SM, Piper PW, Pearl LH (August 2000). "The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains". EMBO J. 19 (16): 4383–92.

Gene Name :

HSP90AA1

Protein Name :

Heat shock protein HSP 90-alpha

Gene Full Name :

heat shock protein 90kDa alpha(cytosolic), class A member 1

Synonyms :

Heat shock 86 kDa antibody Heat shock 90kDa protein 1 alpha antibody Heat shock protein 90kDa alpha(cytosolic) class A member 1 antibody heat shock protein 90kDa alpha(cytosolic), class A member 2 antibody Heat shock protein HSP 90-alpha antibody HSP 86 antibody HSP86 antibody Hsp89 antibody HSP89A antibody HSP90A antibody HSP90AA1 antibody HSP90ALPHA antibody HSP90N antibody HSPC1 antibody HSPCA antibody HSPCAL1 antibody HSPCAL3 antibody HSPCAL4 antibody HSPN antibody LAP2 antibody Renal carcinoma antigen NY REN 38 antibody Renal carcinoma antigen NY-REN-38 antibody

Uniprot ID :

P07900

more info or order :

Boster Immunoleader product webpage