Boster Immunoleader

antibody

Anti-SMAD 1/2/3/5 Antibody

PA1331

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-SMAD 1/2/3/5 Antibody

PA1331

Polyclonal

Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 1(SMAD1) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human SMAD 1/2/3/5(442-456aa GPLQWLDKVLTQMGS), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-beta superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.

1. Zhu, H., Kavsak, P., Abdollah, S., Wrana, J. L., Thomsen, G. H. A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature 400: 687-693, 1999.

SMAD1

Mothers against decapentaplegic homolog 1(MAD homolog 1/Mothers against DPP homolog 1)

SMAD family member 1

BSP 1 antibody BSP1 antibody DKFZP586N0721 antibody DKFZp686J10186 antibody Dwarfin A antibody DwfA antibody hMAD 2 antibody hMAD 3 antibody hSMAD2 antibody hSMAD3 antibody hSmad5 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV152 antibody JV18 1 antibody JV18 antibody JV181 antibody JV4 1 antibody MAD(mothers against decapentaplegic Drosophila) homolog 3 antibody MAD antibody MAD homolg 5 antibody MAD mothers against decapentaplegic homolog 1 antibody Mad related protein 1 antibody MAD Related Protein 2 antibody MAD3 antibody MADH 1 antibody MADH 3 antibody MADH1 antibody MADH2 antibody MADH3 antibody MADR1 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody MGC60396 antibody Mothers against decapentaplegic homolog 1 antibody Mothers Against Decapentaplegic Homolog 2 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against decapentaplegic homolog 5 antibody Mothers against DPP homolog 1 antibody Mothers against DPP homolog 2 antibody Mothers against DPP homolog 3 antibody Mothers against DPP homolog 5 antibody Sma and Mad related protein 1 antibody SMA and MAD related protein 3 antibody SMAD antibody SMAD 1 antibody SMAD 2 antibody SMAD 3 antibody SMAD 5 antibody SMAD family member 1 antibody SMAD mothers against DPP homolog 1 Drosophila antibody Spinal muscular atrophy distal with upper limb predominance antibody TGF beta signaling protein 1 antibody Transforming growth factor beta signaling protein 1 antibody

Q15797