Anti-Actin Antibody | Boster Immunoleader PA1324 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Actin Antibody

catalog :

PA1324

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed publisher
Xie S, Xie N, Li Y, Wang P, Zhang C, Li Q, et al. Upregulation of TRB2 induced by miR-98 in the early lesions of large artery of type-2 diabetic rat. Mol Cell Biochem. 2012;361:305-14 pubmed publisher

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Boster Immunoleader product webpage

product information

Availability :

info changed 20150507

Product Name :

Anti-Actin Antibody

Catalog Number :

PA1324

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Actin, cytoplasmic 2(ACTG1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Liquid

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences.

Contents :

Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification :

Immunogen affinity purified.

Storage :

At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Reference :

1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene.J. Child Neurol.2004; 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

Gene Name :

ACTG1

Protein Name :

Actin, cytoplasmic 2

Gene Full Name :

actin, gamma 1

Synonyms :

Gamma-actin

Uniprot ID :

P63261

more info or order :

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