Anti-NPHS2 Antibody | Boster Immunoleader PA1322 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-NPHS2 Antibody

catalog :

PA1322

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-NPHS2 Antibody

Catalog Number :

PA1322

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Podocin(NPHS2) detection. Tested with WB in Human;Mouse;Rat(Kidney).

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human, mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Podocin(PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al.(2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.

Reference :

1. Boute, N.; Gribouval, O.; Roselli, S.; Benessy, F.; Lee, H.; Fuchshuber, A.; Dahan, K.; Gubler, M.-C.; Niaudet, P.; Antignac, C. : NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. Nature Genet. 24: 349-354, 2000. Note: Erratum: Nature Genet. 25: 125 only, 2000.

Gene Name :

NPHS2

Protein Name :

Podocin

Gene Full Name :

nephrosis 2, idiopathic, steroid-resistant(podocin)

Synonyms :

Nephrosis 2, idiopathic, steroid resistant(podocin) antibody nephrosis 2, idiopathic, steroid resistant antibody NPHS2 antibody NPHS2 gene antibody PDCN antibody PODO_HUMAN antibody Podocin antibody SRN1 antibody

Uniprot ID :

Q9NP85

more info or order :

Boster Immunoleader product webpage