Boster Immunoleader

antibody

Anti-TrkA Antibody

PA1321

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

info changed 20150427

Anti-TrkA Antibody

PA1321

Polyclonal

Rabbit IgG polyclonal antibody for High affinity nerve growth factor receptor(NTRK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence in the middle region of human TrkA(342-361aa RHGCLRLNQPTHVNNGNYTL), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Trk A(Neurotrophic tyrosine kinase receptor A) is the high affinity catalytic receptor for the neurotrophin, Nerve Growth Factor(NGF). Higher affinity binding of NGFR can achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA(NTRK1), TRKB(NTRK2), and TRKC(NTRK3). TRKA, TRKB, and TRKC are specific for or "preferred by" NGF, NTF4(Neurotrophin-4) and BDNF, and NTF3(Neurotrophin-3), respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity. The absence of TrkA(NGFR) expression was associated with a strong increase in the Sp3 repressor short isoform(s) and a lack of the Sp3 activator long isoform. Sp3 is a bifunctional transcription factor that has been reported to stimulate or repress the transcription of numerous genes. Indo et al.(1996) concluded that defects in TRKA cause CIPA(Congenital insensitivity to pain with anhidrosis) and that the NGF-TRKA system has a crucial role in the development and function of the nociceptive reception as well as establishment of thermoregulation via sweating in humans. These results also implicate genes encoding other TRK and neurotrophin family members as candidates for developmental defect(s) of the nervous system.

1. Lambiase A, Merlo D, Mollinari C, Bonini P, Rinaldi AM, D' Amato M, Micera A, Coassin M, Rama P, Bonini S, Garaci E (November 2005). "Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3". Proc. Natl. Acad. Sci. U.S.A.102 (46): 16795–800. 2. Indo, Y.; Tsuruta, M.; Hayashida, Y.; Karim, M. A.; Ohta, K.; Kawano, T.; Mitsubuchi, H.; Tonoki, H.; Awaya, Y.; Matsuda, I. : Mutations in the TRKA/NGF receptor gene in patients with congenital insensitivity to pain with anhidrosis. Nature Genet. 13: 485-488, 1996.

NTRK1

High affinity nerve growth factor receptor

neurotrophic tyrosine kinase, receptor, type 1

CIPA antibody DKFZp781I14186 antibody gp140trk antibody High affinity nerve growth factor receptor antibody High affinity nerve growth factor receptor precursor antibody MTC antibody neurotrophic tyrosine kinase receptor type 1 antibody Ntrk 1 antibody Ntrk1 antibody NTRK1_HUMAN antibody Oncogene TRK antibody p140 TrkA antibody p140-TrkA antibody TRK 1 antibody Trk A antibody TRK antibody Trk-A antibody TRK1 antibody TRK1 transforming tyrosine kinase protein antibody TRK1-transforming tyrosine kinase protein antibody Tropomyosin-related kinase A antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody Tyrosine Receptor Kinase A antibody

P04629