Anti-PPAR gamma Antibody | Boster Immunoleader PA1320 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-PPAR gamma Antibody

catalog :

PA1320

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

publications citing this reagent :

Xie W, Wang H, Zhang J, Li J, Can Y, Qing L, et al. Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice. Exp Ther Med. 2011;2:309-315 pubmed

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product information

Product Name :

Anti-PPAR gamma Antibody

Catalog Number :

PA1320

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor gamma(PPARG) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma(45-62aa PHYEDIPFTRTDPVVADY), different from the rat and mouse sequences by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

The peroxisome proliferator-activated receptors(PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements(PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al.(2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

Reference :

1. Berger J, Moller DE (2002). "The mechanisms of action of PPARs". Annu. Rev. Med. 53: 409–35. 2. Deeb, S. S.; Fajas, L.; Nemoto, M.; Pihlajamaki, J.; Mykkanen, L.; Kuusisto, J.; Laakso, M.; Fujimoto, W.; Auwerx, J. : A pro12ala substitution in PPAR-gamma-2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity. Nature Genet. 20: 284-287, 1998. 3. Ameshima, S.; Golpon, H.; Cool, C. D.; Chan, D.; Vandivier, R. W.; Gardai, S. J.; Wick, M.; Nemenoff, R. A.; Geraci, M. W.; Voelkel, N. F. : Peroxisome proliferator-activated receptor gamma (PPAR-gamma) expression is decreased in pulmonary hypertension and affects endothelial cell growth. Circ. Res. 92: 1162-1169, 2003.

Gene Name :

PPARG

Protein Name :

Peroxisome proliferator-activated receptor gamma(PPAR-gamma)

Gene Full Name :

peroxisome proliferator-activated receptor gamma

Synonyms :

CIMT1 antibody GLM1 antibody HUMPPARG antibody NR1C3 antibody Nuclear receptor subfamily 1 group C member 3 antibody OTTHUMP00000185032 antibody OTTHUMP00000185036 antibody PAX8/PPARG Fusion Gene antibody Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody Peroxisome proliferator activated receptor gamma 1 antibody Peroxisome Proliferator Activated Receptor gamma antibody Peroxisome proliferator-activated receptor gamma antibody PPAR gamma antibody PPAR-gamma antibody PPARG antibody PPARG_HUMAN antibody PPARG1 antibody PPARG2 antibody PPARG3 antibody

Uniprot ID :

P37231

more info or order :

Boster Immunoleader product webpage