Boster Immunoleader

antibody

Anti-SNAP25 Antibody

PA1315

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-SNAP25 Antibody

PA1315

Polyclonal

Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: rat Predicted to work with: human, mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Synaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE(the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa(SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E(BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms(a and b) have been described, which are produced by alternative splicing. Nagy et al.(2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

1. Gonelle-Gispert, C.; Halban, P. A.; Niemann, H.; Palmer, M.; Catsicas, S.; Sadoul, K. : SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion. Biochem. J. 339: 159-165, 1999. 2. Nagy, G.; Reim, K.; Matti, U.; Brose, N.; Binz, T.; Rettig, J.; Neher, E.; Sorensen, J. B. : Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron 41: 417-429, 2004. 3. Hodel A (1998). "SNAP-25". The International Journal of Biochemistry & Cell Biology 30 (10): 1069–1073. 4. Chapman ER (2002). "Synaptotagmin: A Ca2+ sensor that triggers exocytosis?". Nature Reviews Molecular Cell Biology 3: 498–508. 5. Pozzi D, Verderio C, Patti L, Grumelli C, Inverardi F, Frassoni C, Bonanno G, Matteoli M (2004). "SNAP-25 modulation of calcium dynamics underlies differences in GABAergic and glutamatergic responsiveness to depolarization". Neuron 41 (4): 599–610.

SNAP25

Synaptosomal-associated protein 25(SNAP-25)

synaptosomal-associated protein, 25kDa

bA416N4.2 antibody Bdr antibody dJ1068F16.2 antibody FLJ23079 antibody HGNC:11132 antibody MGC105414 antibody MGC139754 antibody Resistance to inhibitors of cholinesterase 4 homolog antibody RIC 4 antibody RIC4 antibody SEC 9 antibody SEC9 antibody SNAP 25 antibody SNAP antibody SNAP-25 antibody SNAP-25B antibody SNAP25 antibody SNP 25 antibody SNP25 antibody SNP25_HUMAN antibody sp antibody SUP antibody Super protein antibody Synaptosomal associated 25 kDa protein antibody Synaptosomal associated protein antibody Synaptosomal Associated Protein 25 antibody Synaptosomal associated protein 25kDa antibody Synaptosomal-associated 25 kDa protein antibody Synaptosomal-associated protein 25 antibody Synaptosomal-associated protein, 25-KD antibody

P60880