Anti-ERK2 Antibody | Boster Immunoleader PA1310 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ERK2 Antibody

catalog :

PA1310

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

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product information

Availability :

info changed 20150427

Product Name :

Anti-ERK2 Antibody

Catalog Number :

PA1310

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 1(MAPK1) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2(348-360aa FEETARFQPGYRS), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.

Reference :

1. Owaki H, Makar R, Boulton TG, Cobb MH, Geppert TD (February 1992). "Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs". Biochem. Biophys. Res. Commun. 182 (3): 1416–22. 2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein kinases. Oncogene 9: 647-649, 1994. 3. Owaki, H.; Makar, R.; Boulton, T. G.; Cobb, M. H.; Geppert, T. D. : Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs. Biochem. Biophys. Res. Commun. 182: 1416-1422, 1992. 4. Khokhlatchev, A. V.; Canagarajah, B.; Wilsbacher, J.; Robinson, M.; Atkinson, M.; Goldsmith, E.; Cobb, M. H. : Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation. Cell 93: 605-615, 1998. 5. Di Cristo, G.; Berardi, N.; Cancedda, L.; Pizzorusso, T.; Putignano, E.; Ratto, G. M.; Maffei, L. : Requirement of ERK activation for visual cortical plasticity. Science 292: 2337-2340, 2001. 6. Forcet, C.; Stein, E.; Pays, L.; Corset, V.; Llambi, F.; Tessier-Lavigne, M.; Mehlen, P. : Netrin-1-mediated axon outgrowth requires deleted in colorectal cancer-dependent MAPK activation. Nature 417: 443-447, 2002.

Gene Name :

MAPK1

Protein Name :

Mitogen-activated protein kinase 1(MAP kinase 1/MAPK 1)

Gene Full Name :

mitogen-activated protein kinase 1

Synonyms :

ERK 2 antibody ERK antibody ERK-2 antibody ERT 1 antibody ERT1 antibody Extracellular Signal Regulated Kinase 2 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAPK 1 antibody MAPK 2 antibody MAPK 2 antibody MAPK1 antibody MAPK2 antibody Mitogen Activated Protein Kinase 1 antibody Mitogen Activated Protein Kinase 2 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody P38 antibody P40 antibody P41 antibody P41MAPK antibody p42 MAPK antibody p42-MAPK antibody P42MAPK antibody P42MAPK antibody PRKM 1 antibody PRKM 2 antibody PRKM1 antibody PRKM2 antibody Protein Tyrosine Kinase ERK2 antibody

Uniprot ID :

P28482

more info or order :

Boster Immunoleader product webpage