Boster Immunoleader

antibody

Anti-SDHA Antibody

PA1306

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

info changed 20150507

Anti-SDHA Antibody

PA1306

Polyclonal

Rabbit IgG polyclonal antibody for Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial(SDHA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Liquid

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human SDHA(641-656aa YRPVIDKTLNEADCAT), identical to the related rat and mouse sequences.

Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.

Immunogen affinity purified.

At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit(SDHA), the iron sulfur protein subunit(SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase(SDH) protein complex catalyzes the oxidation of succinate(succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes(Types I and II) in the paraganglia system.

1. Briere, JJ et al. 2005. Human Molecular Genetics. Vol. 14 pg. 3263-3269.

SDHA

Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial

succinate dehydrogenase complex, subunit A, flavoprotein(Fp)

CMD1GG antibody DHSA_HUMAN antibody Flavoprotein subunit of complex II antibody FP antibody PGL5 antibody SDH 2 antibody SDH1 antibody SDH2 antibody SDHA antibody SDHF antibody Succinate dehydrogenase[ubiquinone] flavoprotein subunit antibody Succinate dehydrogenase[ubiquinone] flavoprotein subunit mitochondrial antibody Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial antibody Succinate dehydrogenase complex flavoprotein subunit antibody Succinate dehydrogenase complex flavoprotein subunit precursor antibody Succinate dehydrogenase complex subunit A antibody Succinate dehydrogenase complex subunit A flavoprotein antibody

P31040