Anti-S100 beta Antibody | Boster Immunoleader PA1303 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-S100 beta Antibody

catalog :

PA1303

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

publications citing this reagent :

Wang Y, Wang K, Chao R, Li J, Zhou L, Ma J, et al. Neuroprotective effect of vaccination with autoantigen-pulsed dendritic cells after spinal cord injury. J Surg Res. 2012;176:281-92 pubmed publisher
Chen P, Chen R, Yang Y, Yu Y, Xie Y, Zou Y, et al. Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium. Int Immunopharmacol. 2009;9:1304-12 pubmed publisher
Cao L, Zhu Y, Su Z, Lv B, Huang Z, Mu L, et al. Olfactory ensheathing cells promote migration of Schwann cells by secreted nerve growth factor. Glia. 2007;55:897-904 pubmed

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product information

Availability :

info changed 20150427

Product Name :

Anti-S100 beta Antibody

Catalog Number :

PA1303

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Protein S100-B(S100B) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta(64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.

Reference :

1. Wainwright, M. S.; Craft, J. M.; Griffin, W. S. T.; Marks, A.; Pineda, J.; Padgett, K. R.; Van Eldik, L. J. : Increased susceptibility of S100B transgenic mice to perinatal hypoxia-ischemia. Ann. Neurol. 56: 61-67, 2004. 2. Reeves, R. H.; Yao, J.; Crowley, M. R.; Buck, S.; Zhang, X.; Yarowsky, P.; Gearhart, J. D.; Hilt, D. C. : Astrocytosis and axonal proliferation in the hippocampus of S100b transgenic mice. Proc. Nat. Acad. Sci. 91: 5359-5363, 1994.

Gene Name :

S100B

Protein Name :

Protein S100-B

Gene Full Name :

S100 calcium binding protein B

Synonyms :

NEF antibody Protein S100 B antibody Protein S100-B antibody S 100 calcium binding protein beta chain antibody S 100 protein beta chain antibody S 100 protein beta subunit antibody S-100 protein beta chain antibody S-100 protein subunit beta antibody S100 antibody S100 calcium binding protein B antibody S100 calcium binding protein beta(neural) antibody S100 calcium binding protein beta chain antibody S100 calcium-binding protein B antibody S100 protein beta chain antibody S100B antibody S100B_HUMAN antibody S100beta antibody

Uniprot ID :

P04271

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